BOXSHADE is a program for pretty-printing multiple alignment output. The program itself doesn't do any alignment, you have to use a multiple alignment program like ClustalW or Pileup and use the output of these programs as input for BOXSHADE. Of course, you can also use manually aligned sequences (in a proper format). INPUT = aligned sequence files.
126742336 -------ATGGTGCTGTCTCCCGCTGACAAGGCCAACGTCAAGGCCGCCTGGGGCAAGGT
110005943 ACCCACCATGGTGCTGTCTGCCGCCGACAAGTCCAATGTCAAGGCCGCCTGGGGCAAGGT
66865969 -------ATGGTGCTGTCTGCCGCCGACAAGTCCAATGTCAAGGCCGCCTGGGGCAAGGT
************ **** ****** **** ***********************
126742336 CGGGGGCCACGCCGGCGAGTATGGCGCCGAGGCCCTGGAGAGG-----------------
110005943 TGGCGGCAACGCTGGAGCTTATGGCGCAGAGGCTCTGGAGAGG-----------------
66865969 TGGCGGCAACGCTCCAGCTTTTGGCGCAGAGGCCCTGGAGAGGTGAGCACCGCACCTGCC
** *** **** * * ****** ***** *********
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